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Immunological Methods for the Detection of a Coryneform Isolated from Ratoon Stunted Sugarcane
Author(s) -
Oellermann R. A.,
Pillay D.
Publication year - 1987
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1987.tb00432.x
Subject(s) - agglutination (biology) , antiserum , biology , hemagglutination , microbiology and biotechnology , bacteria , immunofluorescence , virology , antibody , immunology , genetics
A coryneform bacterium, isolated from ratoon stunted sugarcane, has been obtained in pure culture. Its identification by immunological means was investigated. Optimum conditions for the light microscopic observation of bacterial agglutination by specific antiserum, immunofluorescence and the microcapillary haemagglutination of IgG‐sensitized erythrocytes by the bacterium were established. All three assay procedures are simple and rapid. By comparison, more samples can be handled at any one time bythe microcapillary haemagglutination method. Based on bacterial agglutination, the antiserum is specific for the coryneform isolated from ratoon‐stunted sugarcane.