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Nutzung von In‐vitro ‐Pflanzen der Kartoffel zur Prüfung der antiphytoviralen Wirkung von 2,4‐Dioxohexahydro‐1,3,5‐triazin (DHT) in Kombination mit N‐Cyanoguanidin
Author(s) -
Bittner H.,
Schenk G.,
Schuster G.
Publication year - 1986
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1986.tb04376.x
Subject(s) - in vitro , explant culture , biology , virus , triazine , horticulture , botany , chemistry , biochemistry , virology , organic chemistry
Suitability of in vitro potato plantlets for testing of antiphytoviral effect of a combination of 2,4‐dioxo‐hexahydro‐1,3,5‐triazine (DHT) and N‐cyano guanidine The suitability of a semiquantitative determination of virus content for the detection of antiphytoviral effects of chemicals was demonstrated by ELISA directly applying at in vitro potato cultures systemically infected and chemotherapeutically treated. The combination of 2, 4‐dioxohexahydro‐1,3,5‐triazine (DHT) and N‐cyanoguanidine both applied at a concentration of 0.03% to the culture medium resulted in a high significant reduction of the relative concentration of the potato virus S in the, potato genotype ‘M‐812820’ of 44–73 %. A phytotoxic influence of the substances was not observed. The combination of the two antiphytoviral substances had no effect against potato virus Y in explants of the variety ‘Ackersegen’. Differences in the susc, eptibility of various genotypes against biologically active substances were indicated.

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