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Production of Cutin Hydrolyzing Enzymes by Botrytis cinerea in vitro
Author(s) -
Salinas J.,
Warnaar F.,
Verhoeff K.
Publication year - 1986
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1986.tb00924.x
Subject(s) - cutin , cutinase , botrytis cinerea , biology , hydrolysis , enzyme , biochemistry , food science , botany
The production in vitro of cutin hydrolyzing enzymes by five isolates of B. cinerea was studied, using cutin of tomato fruits as a carbon source. Chemical depolymerisation of the cutin yielded 10,16‐dihydroxyhexadecanoic acid as the main component. The same fatty acid was found after incubation of cutin with a crude enzyme preparation from a culture filtrate of B. cinerea. Hydrolysis was optimal at pH 5.5–6.0. In cultures with glucose as the only carbon source no cutinase activity was detected. Crude enzyme preparations which hydrolyzed cutin, also hydrolyzed para‐nitrophenylbutyrate, with an optimum activity at pH 8. All five isolates showed para‐nitrophenylbutyrate hydrolyzing activity when grown on tomato cutin, but the activity varied with the isolate used. No correlation was found between para‐nitrophenylbutyrate‐hydrolyzing activity of an isolate and its production of small lesions on young tomato fruits.