Premium
The Search for a Specific Antigen for Erwinia amylovora
Author(s) -
Laroche M.,
Verhoyen M.
Publication year - 1986
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1986.tb00920.x
Subject(s) - erwinia , biology , antigen , centrifugation , immunodiffusion , microbiology and biotechnology , bacteria , enterobacteriaceae , fire blight , biochemistry , escherichia coli , immunology , gene , genetics
A specific reaction has been observed in immunodiffusion, using the supernatant obtained after centrifugation of E. amylovora phenol treated cells. This reaction occurred for all the E. amylovora strains examined and could be distinguished from all other reactions occurring with different species of Erwinia. We called the product inducing the specific reaction: Specific Antigenic Fraction or SAF. The immunological and chemical characteristics of SAF have been compared with other extractable products from the bacteria (LPS, ‘O'Ag), from the culture media (EPSc) and from the ooze (EPSo). This revealed that SAF is lipopolysaccharide in nature and that the specific antigen(s) involved would be on a polysaccharidic fraction.