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Interrelationship between Nutrients, Pathogenicity and Phytoalexin Metabolism of Botrytis cinerea on Clover Leaves
Author(s) -
Macfoy C. A.,
Smith I. M.
Publication year - 1986
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1986.tb00911.x
Subject(s) - phytoalexin , botrytis cinerea , spore , mycelium , biology , inoculation , sucrose , incubation , botany , horticulture , microbiology and biotechnology , food science , biochemistry , resveratrol
Botrytis cinerea spores suspended in 0.28 M glucose solution caused limited lesions on clover leaves, on which the clover phytoalexins maackiain and medicarpin accumulated to 1028 μg and 856 μg/g fresh wt respectively after 4 days incubation. During this time, little or none of the phytoalexin degradation products were detected. On the other hand, B. cinerea spores in sucrose casamino acids (SCA) liquid medium caused larger lesions than spores in glucose, and less maackiain and medicarpin (298 μg and 95 μg/g fresh wt respectively) and high concentrations of the degradation products were detected. B. cinerea mycelium in SCA also caused large lesions and both the phytoalexins and their degradation products were detected., Sclerotinia laxa spores in 0.28 M glucose or its mycelium in SCA liquid medium did not cause any lesions apart from a minute necrotic fleck, and although phytoalexins were recovered from leaves inoculated with spores (67 μg and 78 μg/g fresh weight of maackiain and medicarpin respectively after 4 days) and leaves inoculated with mycelium (150 μg and 167 μg/g fresh wt maackiain and medicarpin respectively after 3 days), no phytoalexin degradation products were detected. The implications of, these results in understanding the interrelationship between nutrients, pathogenicity and phytoalexin metabolism are discussed.