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Elimination in vitro of two Grapevine Nepoviruses by an Alternating Temperature Regime *
Author(s) -
Monette P. L.
Publication year - 1986
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1986.tb00898.x
Subject(s) - biology , nepovirus , shoot , in vitro , virology , horticulture , virus , plant virus , botany , genetics
A 40 day in vitro treatment with 6 h at 39°C followed by 18 h at 22°C was effective in eliminating both grapevine fanleaf virus (GFLV) and arabis mosaic virus (AMV) from the developing shoot tips (2 mm) of grapevine shoot tip cultures. Longer treatment durations with consecutive 12 h periods at 35°C and 22°C eliminated GFLV in some cases, but did not eliminate AMV.