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The Action of Polygalacturonase and Cellulase Enzymes of Botryodiplodia theobromae Pat. on Yam ( Dioscorea spp.) and Sweet Potato ( Ipomoea batatas ) Tissues
Author(s) -
Arinse A. E.
Publication year - 1985
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1985.tb00847.x
Subject(s) - cellulase , pectinase , biology , ipomoea , botany , dioscorea , enzyme , biochemistry , pathology , medicine , alternative medicine
The action of an endo‐polygalacturonase PG3 and cellulase of Botryodiplodia theobromae , in the crude and pure forms, on sweet potato and yam slices was investigated. The purified isoenzyme PG3 and the crude pectic enzyme preparations macerated and killed the cells of both tissues although the former did so at a faster rate. Purified cellulase neither macerated nor killed cells from either plant. Although the action was delayed, the crude preparation of cellulase slightly macerated and killed the cells of sweet potato. Yam tissue was, however, not macerated by crude cellulase. Plasmolysis of the cells of both tissues delayed the toxicity and macerating action of the pure PG3 on the tissues. This delay in cell death was, however, not observed when the plasmolysed cells were treated with the crude cellulase preparation.