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Scanning Electron Microscopy of the Influence of Fixation Vehicle Osmolality on Cell Morphology of Spiroplasma, Acholeplasma, and Mycoplasma spp. grown on Agar
Author(s) -
Leeuw G. T. N.,
Vught P. A. M.,
Samson R. A.,
PolakVogelzang A. A.
Publication year - 1985
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1985.tb00836.x
Subject(s) - spiroplasma , glutaraldehyde , biology , microbiology and biotechnology , mycoplasma , sucrose , agar , ultrastructure , distilled water , mollicutes , botany , bacteria , biochemistry , chromatography , chemistry , genetics
Young Spiroplasma citri , corn stunt spiroplasma, and honey bee spiroplasma colonies fixed in 5% glutaraldehyde in M 199 cell culture medium with 0.25 M sucrose showed elongated mycelium‐like cells which were sometimes branched or helical. In older colonies beaded chains and rounded bodies were formed. Fixation in 6 % glutaraldehyde in distilled water resulted in amorphous masses in which rounded bodies were present. The spiroplasma cells did not remain osmotically active after glutaraldehyde fixation. Acholeplasma laidlawii and Mycoplasma hyorhinis colonies fixed in glutaraldehyde with or without M 199 medium with 0.25 M sucrose showed little difference in cell morphology.