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Resistance Mechanism of Cultured Plant Cells to Tobacco Mosaic Virus
Author(s) -
Toyoda H.,
Oishi Y.,
Matsuda Y.,
Chatani K.,
Hirai T.
Publication year - 1985
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1985.tb00834.x
Subject(s) - callus , tobacco mosaic virus , subculture (biology) , biology , somaclonal variation , tissue culture , botany , virus , nicotiana tabacum , virology , in vitro , genetics , gene
In somaclonal tissues obtained from systemically TMV‐infected tobacco plants, a relation between changes of TMV amounts and the callus growth was examined. The culture medium was suitable for maintaining a constant concentration of TMV as well as active callus growth. By using the shake‐culture method, somaclonal tissues were separated into two classes on the basis of callus sizes. In large callus tissues, TMV amounts were constant during subculturing but the tissues did not either grow or release the newly divided cells after the last subculture. On the other hand, smaller callus tissues grew markedly and the TMV amounts were conspicuously lowered. After shake‐subculture of smaller tissues, they were successfully regenerated to plantlets. None of the plantlets expressed any mosaic symptoms, while plantlets from the original somaclones showed severe mosaic symptoms of TMV in leaflets. Thus, the present report describes the successful production of virus‐free plantlets from infected somaclonal callus cultures.