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Sugar‐specific Attachment of Pseudomonas syringae pv. glycinea to Isolated Single Leaf Cells of Resistant Soybean Cultivars
Author(s) -
ÉRsek T.,
Gáborjáanyi R.,
Höltzl P.,
Király Z.
Publication year - 1985
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1985.tb00084.x
Subject(s) - biology , pseudomonas syringae , cultivar , inoculation , sugar , bacteria , botany , horticulture , biochemistry , genetics
Phase‐contrast and scanning electron microscopy showed races of P. synngae pv. glycinea uniformly distributed over and attached to the whole surface of isolated single leaf cells of resistant soybean cultivars, as early as 30 to 180 min after inoculation. On the contrary, attachment in the compatible interaction did not occur within 10—15 h. In a later period, compatibility was characterized by the formation of adherent bacterial clusters. Early attachment of races 1 and 6 to cv. Harosoy and that of race 5 to cv. Flambeau leaf cells, each representing incompatible interaction, could be inhibited by L‐rhamnose and D‐glucose, respectively. Furthermore, the lack of Mn 2+ and Fe 2+ and heat‐treatment of plant cells also affected the early attachment in incompatible combinations and resulted in cluster formation, suggesting incompatibility rather than compatibility to be the active phenomenon. Pre‐inoculation of cells with an incompatible race induced changes that caused compatible bacteria also to distributively attach to plant cell surface indicating that a transfer of information or surface alterations occur upon attachment in incompatible interaction.