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A Technique for Continuous Observation of the Infection Process in Cucumber Anthracnose
Author(s) -
Stumm D.,
Gessler C.
Publication year - 1984
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1984.tb00775.x
Subject(s) - biology , penetration (warfare) , differential interference contrast microscopy , oil immersion , lens (geology) , microscopy , magnification , agar , botany , optics , mathematics , paleontology , physics , genetics , operations research , bacteria
A new light microscope preparation technique for high magnification observation of living plant tissue and fungal penetration is described. Agar immersion is used in differential interference contrast microscopy (DIC) instead of coverslips (lens 40) or instead of coverslips and oil (lens 100). This technique is suitable for (a) longtime observation of living tissue, because the tissue to be observed remains on the plant, and for (b) thick and uneven samples, as no coverslips are required. With this technique it was possible to observe the dynamics of penetration of Colletotrichum lagenarium into epideral cells of cucumber cotyledons for 72 hours. A time lapse film using this technique is in preparation.