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Inhibition of the Hypersensitive Reaction in Tobacco Leaves by Extracellular Polysaccharides from Phytopathogenic Pseudomonads *)
Author(s) -
Mazzucchi U.,
ElBanoby F. E.,
Rudolph K.
Publication year - 1984
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1984.tb00762.x
Subject(s) - biology , polysaccharide , extracellular polysaccharide , extracellular , pseudomonas , microbiology and biotechnology , in vitro , nicotiana tabacum , pseudomonadaceae , pseudomonadales , food science , biochemistry , bacteria , gene , genetics
Lipopolysaccharides (pr‐LPS) and extracellular polysaccharides (EPS) were prepated from in vitro ‐cultures of phytopathogenic pseudomonads ( Pseudomonas phaseolicola race 1 and race 2, P. aptata, P. tabaci ) and purified. The preparations were dissolved in water (0.25–2.5 mg/ml) in infiltrated with P. aptata or P. pisi (9 × 10 7 cells/ml). Whereas the hypersensitive rzaction against P. aptata and P. pisi was inhibited by C.25 mg/ml of pr‐LPS in most of tae cases, the same concentration of EPS caused only partial or no inhibition. However, at 2.5 mg/ml most of the EPS‐preparations caused a complete inhibition. The LPS and EPS‐preparations of P. phaseolicola race 1 caused less inhibition than those of race 2.