Premium
Culturing Puccinia coronata on a Cell Monolayer of the Avena sativa Coleoptile
Author(s) -
Mendgen K.,
Dressler Elvira
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1983.tb00583.x
Subject(s) - haustorium , biology , avena , coleoptile , botany , hypha , infectivity , puccinia , host (biology) , mildew , virology , virus , ecology
A culture of Puccinia coronata on an epidermal cell monolayer dissected from the Avena sativa caleoptile is described. To induce fungal development, infection structure formation had to be induced with a heat treatment. The host cells were fed with 1 % sucrose (or glucose or manitol) to sustain fungal growth. Under these conditions, normal development of hyphae, haustoria with haustorial mother cells and uredospores occurred. Uredospores had normal infectivity.