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Mechanism of Genome Expression in a Single‐stranded RNA Virus from the Cultivated Mushroom [Agaricus bisporus (Lange) Imbach]
Author(s) -
Tavantzis M.,
Romaine C. P.,
Smith S. H.
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1983.tb00026.x
Subject(s) - biology , agaricus bisporus , mycovirus , rna , mushroom , virus , gene , virology , rna silencing , microbiology and biotechnology , rna polymerase , biochemistry , botany , rna interference
The single‐stranded RNA genome of a bacilliform virus from the common cultivated mushroom, Agaricus bisporus , was translated in an in vitro rabbit reticulocyte system. Optimal conditions for translation of mushroom bacilliform virus (MBV) RNA were 2.5 mM Mg 2+ , 70 mM K + , pH 7.2, and 60μg/ml RNA. Gel electrophoretic analysis showed that MBV RNA directs the synthesis of two major polypeptides of MW 77,000 and 37,000 and possibly several minor polypeptides (MW 21,000—28,000). An RNA polymerase activity could not be detected in purified virus preparations. The findings support the notion that the MBV genome functions directly as messenger for protein synthesis and further establishes the closer similarity between MBV and viruses of higher plants than mycoviruses.