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Mechanical Transmission of Soil‐borne Barley Yellow Mosaic Virus
Author(s) -
Friedt W.
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1983.tb00023.x
Subject(s) - inoculation , infectivity , biology , hordeum vulgare , potassium , sodium , phosphate , mosaic virus , potassium phosphate , dilution , virus , agronomy , horticulture , plant virus , poaceae , virology , chemistry , biochemistry , chromatography , physics , organic chemistry , thermodynamics
Leaves of winter barley ( Hordeum vulgare L.) cv. Gerbel were mechanically sap‐inoculated with Barley Yellow Mosaic Virus (BaYMV). Different additives to the inoculation fluid were tested. Whereas the dilution of plant sap by water alone resulted in an infection rate of 43%, the addition of sodium sulphite (80 %) and potassium phosphate‐buffer (89%) increased the proportion of infected plants substantially. Infectivity was further increased by repeated inoculation, when sodium sulphite yielded 96 % and potassium phosphate 100% of infection. The usefulness of the technique in further research and application is discussed.