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Acidic Lugol’s solution as an effective fixative for flounder eggs
Author(s) -
EngellSørensen K.,
Holmstrup M.,
Leonhard S. B.
Publication year - 2012
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2011.01884.x
Subject(s) - fixative , biology , flounder , fish <actinopterygii> , fixation (population genetics) , fishery , biochemistry , cytoplasm , gene
Summary Fixatives used for preservation of marine fish eggs must maintain the structural integrity of eggs in order to secure determination of developmental stages. Formalin is commonly used as a fixative, however, this compound is associated with health issues, and fish eggs fixated in formalin are often not stageable after longer storage. The present study shows that 5% acidic Lugol’s solution is an efficient fixative for ichtyoplankton, and superior to formalin fixation. Flounder eggs retained their structure and staging was easy even after 1 year of storage in acidic Lugol’s solution.