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Detection of SJNNV and RGNNV genotypes using a relative quantification RT‐PCR assay
Author(s) -
Cherif N.,
LopezJimena B.,
GarciaRosado E.,
Cano I.,
Castro D.,
Borrego J. J.,
Alonso M. C.,
Hammami S.
Publication year - 2011
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2010.01592.x
Subject(s) - biology , virology , sea bass , genotype , virus , asymptomatic , aquaculture , fish <actinopterygii> , fishery , gene , genetics , medicine
Summary Viral Encephalopathy and Retinopathy (VER), is caused by a nodavirus included within the Betanodavirus genus of the Nodaviridae family. This disease affects more than 30 marine fish species worldwide and has been a major obstacle in the aquaculture industry; control of the disease is based on virus detection, essentially in carrier specimens. This study describes a real time PCR procedure for viral nervous necrosis virus detection from several organs of sea bass, Senegalese sole, and gilt‐head sea bream, from fish displaying either clinical symptoms or asymptomatic cases. The sensitivity of this technique was about 10 6 ‐fold higher than that of the conventional RT‐PCR. The newly designed primers detected nodavirus isolates belonging to the RGNNV and SJNNV genotypes.