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Stomach development in the sturgeon Acipenser naccarii : histoenzymatic and ultrastructural analysis
Author(s) -
Camacho S.,
Carmona R.,
Llorente J. I.,
Sanz A.,
GarcíaGallego M.,
Domezain A.,
Dominguez N.,
OstosGarrido M. V.
Publication year - 2011
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2010.01581.x
Subject(s) - biology , yolk , ultrastructure , yolk sac , stomach , sturgeon , embryogenesis , epithelium , hatching , gastric glands , anatomy , embryo , medicine , microbiology and biotechnology , endocrinology , andrology , gastric mucosa , biochemistry , fishery , fish <actinopterygii> , ecology , genetics
Summary This study describes the stomach development in the sturgeon Acipenser naccarii , focusing on its histoenzymatic and ultrastructural characteristics from hatching until the end of the juvenile phase, 1 month later. The study aims to assist the aquaculturist in identifying the best timing for the start of feeding and to select the appropriate food for each stage of A. naccarii development. At hatching, the anterior portion of the digestive system consists of a yolk sac with a large central yolk mass. The yolk mass is enclosed by differentiating endodermal epithelium. The most significant ultrastructural characteristics of the epithelium are the numerous supranuclear vacuoles containing protein and lipid yolk granules, dilated intercellular spaces, and filiform cytoplasmic projections related to the uptake of reserve material on the apical surface. Newly hatched eleuthero‐embryos show optimal enzymatic activities at a pH of 1.5 and 3, which is probably related to the presence of hatching glands and the digestion of yolk components. During the free‐embryo phase, the yolk sac epithelium undergoes a series of histological, ultrastructural, and functional modifications to form a cecal stomach with anatomical characteristics similar to those of juvenile specimens. Gastric glands emerge between 6 and 7 days post‐hatching (PH), although the absence of secretion granules in their oxyntic‐peptic cells indicates that the stomach is not yet functional. During the following days (the exotrophic period), development of the tubular–vesicular network is observed, and secreting granules occur in the cytoplasm of oxyntic‐peptic cells until around day 35 after fertilization (day 27 PH), when acid protease enzymatic activity is again detected. These histological and enzymatic changes and the greater number and size of oxyntic‐peptic cell secretion granules appear to be related to the gastric compartment ontogeny reaching functionality at this developmental stage.