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Physico‐biochemical parameters and protein profiles of sperm from beluga Huso huso
Author(s) -
Li P.,
Rodina M.,
Hulak M.,
Gela D.,
Li Z. H.,
Linhart O.
Publication year - 2010
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2010.01544.x
Subject(s) - huso , beluga , biology , sperm , sturgeon , sperm motility , motility , extender , semen , cryopreservation , reproduction , andrology , spermatogenesis , zoology , anatomy , endocrinology , fishery , chemistry , ecology , botany , embryo , microbiology and biotechnology , medicine , organic chemistry , fish <actinopterygii> , polyurethane
Summary Basic physico‐biochemical parameters and protein profiles of sperm from beluga ( Huso huso ), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 μms −1 ) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml −1 ), spermatozoa concentration (0.28 ± 0.27 × 10 9 spz ml −1 ), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg −1 ), the pH (8.49 ± 0.01) and Na + (18.97 ± 3.65 m m ) , K + (2.83 ± 1.36 m m ), Ca 2+ (0.19 ± 0.06 m m ), Mg 2+ (0.49 ± 0.23 m m ) and Cl − (6.33 ± 0.58 m m ) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and iso‐electric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.