Premium
Synchrotron microcomputer tomography for the non‐destructive visualization of the fish skeleton
Author(s) -
Epple M.,
Neues F.
Publication year - 2010
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2010.01422.x
Subject(s) - danio , oryzias , biology , zebrafish , skeleton (computer programming) , fish <actinopterygii> , synchrotron radiation , anatomy , synchrotron , tomography , visualization , high resolution , optics , computer science , artificial intelligence , fishery , remote sensing , physics , biochemistry , gene , geology
Summary The objective of this study was to elucidate the potential of synchrotron radiation‐based microcomputer tomography as a non‐destructive method to visualize hard tissue in fish with high resolution. The high X‐ray contrast between mineralized tissue (bone and teeth) and soft tissue permits an easy differentiation. The nature of this method avoids preparation artefacts which may occur during the preparation for histology or scanning electron microscopy. The spatial resolution is of the order of a few μm, depending on the sample. Results obtained for the species medaka ( Oryzias latipes ) and zebrafish ( Danio rerio ) are presented.