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Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass ( Dicentrarchus labrax ) spermatozoa
Author(s) -
Peñaranda D. S.,
Pérez L.,
Fakriadis G.,
Mylonas C. C.,
Asturiano J. F.
Publication year - 2008
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2008.01124.x
Subject(s) - sea bass , dicentrarchus , cryoprotectant , biology , motility , sperm motility , glycerol , cryopreservation , bass (fish) , sperm , andrology , anatomy , fishery , biochemistry , fish <actinopterygii> , botany , embryo , medicine , genetics
Summary The aim of this study was to evaluate the effects of different extenders on sea bass ( Dicentrarchus labrax) spermatozoa motility and morphology. Six sperm extenders based on inactivator media, DI 1 (here named SAN) and Non‐Activating Medium (NAM) were tested with European sea bass spermatozoa. The best results were obtained with NAM medium (59.83 m m NaCl, 12.91 m m MgCl 2 , 1.47 m m KCl, 3.51 m m CaCl 2 , 20 m m NaHCO 3 , 0.44 m m glucose) plus 1 and 2% of BSA (NAM1 and NAM2, respectively). The motility of the spermatozoa incubated in those media was similar to the fresh sperm until 48 h (NAM1: 74.3 ± 5.4; NAM2: 78.8 ± 5.8%, and higher than undiluted sperm, 19.1 ± 7.8). We also checked the spermatozoa motility and morphology reactions with some of the best extenders, NAM2 and SAN, and combined them with different concentrations (2, 5, 10%) of three cryoprotectants: methanol, glycerol and dimethyl sulphoxide (DMSO). Glycerol + SAN or NAM2 caused activation of spermatozoa motility, which was lost 5 min later. Methanol and DMSO plus NAM2 extenders resulted in a low activation level and high motility 5 min after incubation, identifying these combinations as good candidates to be used in the cryopreservation of the European sea bass spermatozoa.