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Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila
Author(s) -
Sahu S.,
Das B. K.,
Mishra B. K.,
Pradhan J.,
Sarangi N.
Publication year - 2007
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2006.00785.x
Subject(s) - aeromonas hydrophila , biology , labeo , allium sativum , globulin , lysozyme , albumin , microbiology and biotechnology , blood proteins , immunology , biochemistry , botany , fish <actinopterygii> , fishery
Summary The aim of this study was to evaluate dietary dosages of garlic on the immune response and disease resistance against infections due to the opportunistic pathogen Aeromonas hydrophila in Labeo rohita fingerlings. Garlic, Allium sativum, was incorporated into the diets (0%, 0.1%, 0.5% and 1.0%) of rohu, L. rohita fingerlings (10 ± 2 g). Every 20 days, different biochemical (serum total protein, albumin, globulin, albumin : globulin ratio, blood glucose), haematological (WBC, RBC and haemoglobin content) and immunological (superoxide anion production, lysozyme activity and serum bactericidal activity) parameters were evaluated. Superoxide anion production, lysozyme, serum bactericidal, serum protein and albumin were enhanced in garlic treated groups compared with the control group. After 60 days, fish were challenged with A. hydrophila and mortality (%) was recorded up to day 10 post‐challenge. Survival decreased in control group (57%) up to day 10 after infection. However, this was increased in the garlic treatment group, i.e. 85% survivability in the 1 g garlic kg −1 (B group) and 5 g garlic kg −1 (C group), and 71% survivability in the 10 g garlic kg −1 (D group), respectively. These results indicate that Allium sativum stimulates the immunity and makes L. rohita more resistant to infection by A. hydrophila .