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Complex mutation at a microsatellite locus in sturgeons: Acipenser sinensis, A. schrenckii, A. gueldenstaedtii and A. baerii
Author(s) -
Shao Z.,
Lek S.,
Chang J.
Publication year - 2005
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2004.00629.x
Subject(s) - biology , sturgeon , microsatellite , locus (genetics) , genetics , allele , gene , fishery , fish <actinopterygii>
Summary Cross‐species amplifications of microsatellite locus Spl‐106, which was originally screened from the genome of shovelnose sturgeon ( Scaphirhynchus platorynchus ) with a perfect TAGA repeat motif, were carried out in four other species of the genera Acipenser . A total of 34 polymerase chain reaction (PCR) products representing 16 different alleles of this locus was sequenced. Sequence analysis results showed that besides the number changes of repeat units, many mutational events, such as single‐base substitutions and various insertion/deletion (indels) occurred not only at species level but also at individual level, even among the different alleles within the same individual. The repeat motifs varied from perfect (TAGA)n array to perfect compound (TAAA)m (GAAA)n and perfect or imperfect compound (TAAA)m (TAGA)n (TAAA)x arrays in different species and different individuals. The evolution dynamics of this locus in sturgeons was inferred in that it may evolve from a single perfect to different perfect or imperfect compounds.