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Influence of immunostimulants on the effectiveness of vaccines in fish: in vitro and in vivo study
Author(s) -
Siwicki A. K.,
Morand M.,
TerechMajewska E.,
Niemczuk W.,
Kazuń K.,
Glabski E.
Publication year - 1998
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1998.tb00646.x
Subject(s) - elispot , immune system , biology , in vivo , adjuvant , immunology , humoral immunity , immunization , immunostimulant , immunity , antibody , vaccination , t cell , microbiology and biotechnology
Summary Immunostimulants activate nonspecific defence mechanisms, cell‐mediated immunity and specific immune responses. Immunostimulants can be administered before, with, or after vaccines to amplify the specific immune response in generating elevations of humoral antibody levels and the number of antibody‐secreting cells (ASC). Special applications of immunostimulants include assisting spray or other regimens to increase the topical uptake of vaccines. In the present study, the influence of dimerized lysozyme (KLP‐602) and inosine pranobex (gro‐prinosin) on the cellular and humoral immune response after in vitro and in vivo immunization of rainbow trout with Yersinia ruckeri vaccine (Yersivax) was determined. Immunostimulants were administered by injection before, with, or after the vaccine applied by immersion. ELISPOT assays were used for the quantification of total and specific ASC after in vitro and in vivo immunization. Also, the specific immune response was determined 7, 14, 18, 21, 28, 35 and 45 days after in vivo immunization. Immunostimulants administered before, with, or after the vaccine increased cell‐mediated immunity and the specific immune response, compared with the control groups (vaccine only). The higher responses (adjuvant effect) after the application of dimerized lysozyme (KLP‐602) were observed in each experimental study.