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Haematology of swordtail, Xiphophorus helleri. II: cellular composition and isolation of blood cells from kidney of Xiphophorus helleri
Author(s) -
Hamers R.,
Lehmann J.,
Schütt D.A.,
Goerlich R.
Publication year - 1997
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1997.tb00107.x
Subject(s) - xiphophorus , biology , kidney , haematopoiesis , centrifugation , fish <actinopterygii> , zoology , microbiology and biotechnology , andrology , endocrinology , fishery , biochemistry , stem cell , medicine
The quantitative determination of blood cells in the kidney of swordtail, Xiphophorus helleri , was determined by light microscopical observations. Lymphocytes/thrombocytes and neutro‐philic granulocytes (32.4 and 31.6%, respectively) represent the main blood cells in kidney. High amounts of blastic cells in different mitotic phases indicate the role of the kidney as a major haematopoietic organ in this fish species. Additionally, a good and reproducible method for the isolation of accessory and immunoreactive cells was developed using density gradient centrifugation.