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Escherichia coli ‐produced fish growth hormone as a feed additive to enhance the growth of juvenile black seabream ( Acanthopagrus schlegeli )
Author(s) -
Tsai H.J.,
Hsih M.H.,
Kuo J.C.
Publication year - 1997
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1997.tb00105.x
Subject(s) - biology , juvenile , sparidae , escherichia coli , fish meal , zoology , feed conversion ratio , growth hormone , fishery , fish <actinopterygii> , body weight , hormone , biochemistry , endocrinology , ecology , gene
Recombinant growth hormone (rGH) of fish was highly efficiently synthesized by Escherichia coli cells harbouring an expression plasmid (pKLYP) containing a 600 base pairs segment of cDNA which encoded the mature region of yellowfin porgy ( Acanthopagrus latus ) pre‐growth hormone. After dena‐turation and renaturation, rGH purified from inclusion bodies became the refolding form. rGH were then processed into the fishmeal pellet at various concentrations. Juvenile black sea‐bream ( Acanthopagrus schlegeli ) fed twice daily with meal containing 0.5% rGH exhibited significant increases (P < 0.01) in percentage weight gain (60%) and feed efficiency (41%) relative to fish in the control group at week 12. Growth enhancement was maintained for at least 4 weeks (at week 16) after the termination of treatment.