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The influence of various cryoprotectants on semen quality of the rainbow trout ( Oncorhynchus mykiss ) before and after cryopreservation
Author(s) -
Lahnsteiner F.,
Berger B.,
Weismann T.,
Patzner R.
Publication year - 1996
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1996.tb00070.x
Subject(s) - cryoprotectant , extender , rainbow trout , biology , cryopreservation , sperm , glycerol , semen , andrology , sperm motility , lactate dehydrogenase , human fertilization , yolk , trout , zoology , biochemistry , anatomy , food science , botany , chemistry , fishery , enzyme , fish <actinopterygii> , embryo , medicine , organic chemistry , polyurethane
The influence of permeating and of non‐permeating cryoprotectants on motility, eosin permeability (sperm cells viability test) and leaking lactate dehydrogenase activity of spermatozoa of the rainbow trout ( Oncorhynchus mykiss ) was investigated. Correlations between sperm motility rate, percentage of eosinimpermeable (viable) cells and LDH activities established the three parameters as indicators for damages to rainbow trout spermatozoa. High sperm motility rates, velocities, and numbers of linear swimming sperm cells, high numbers of eosinimpermeable spermatozoa and a low extracellular LDH activity were evaluated as positive quality characteristics of semen. With a buffered physiological saline solution as basic extender we found a mixture of 0.67 mol L (5%) dimethylsulfoxide (DMSO) and 0.13 mol/L (1%) glycerol to be the most effective cryoprotectant, followed by DMSO (0.67 mol/L [5%]‐1.85 mol/L [15%]) and glycerol (0.65 mol/L [5%]‐1.78 mol/L [15%]) and propanediol (1.24mol L [10%]‐1.78 mol/L [15%]). Addition of hen egg yolk (7%, 20%) and of 15 mmol/L (0.5%) sucrose significantly increased the semen quality in comparison to the same extender without these additives. Bovine serum albumin (1.5%, 3%) had no influence on the investigated semen parameters.