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Detection of a G/T base substitution in two bleak ( Alburnus alburnus ) populations by RAPD
Author(s) -
Fuchs H.,
Anastassiadis C.,
Schlee P.,
Stein H.,
Rottmann O.
Publication year - 1996
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1996.tb00069.x
Subject(s) - biology , rapd , allele , genomic dna , genetics , zoology , allele frequency , dna , gene , population , demography , sociology , genetic diversity
A G/T substitution has been found in a 462 bp fragment produced by random PCR amplification (RAPD) of genomic DNA in bleak ( Alburnus alburnus ). Two bleak populations from the rivers Main and Danube were screened for the two variants by allele‐specific PCR. The allele frequencies were 0.62 and 0.31 for variant T, and 0.38 and 0.69 for variant G in the river populations of Main and Danube, respectively. Allele frequencies differed significantly (P < 0.01).