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Susceptibility of turbot ( Scophthalmus maximus ), coho salmon ( Oncorhynchus kisutch , and rainbow trout of. my kiss ) to strains of Vibrio anguillarum and their exotoxins
Author(s) -
Santos Ysabel,
Laixier R.,
Bandin Isabel,
Lamas J.,
Toranzo Alicia E.
Publication year - 1991
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1991.tb00522.x
Subject(s) - turbot , vibrio anguillarum , biology , rainbow trout , scophthalmus , virulence , microbiology and biotechnology , trout , serotype , hemolysin , vibrio , fishery , bacteria , fish <actinopterygii> , biochemistry , genetics , gene
Summary The susceptibility of turbot, coho salmon, and rainbow trout to strains of Vibrio anguillarum of serotypes 01 and 02 and their extracellular products (ECP) was investigated in order to clarify the role of exotoxins in the mechanism of virulence of both serotypes. All V. anguillarum isolates were virulent for trout, salmon, and turbot. Despite the origin of the strains tested, rainbow trout was the most susceptible fish species to experimentally induced vibriosis. Coho salmon and turbot did not differ significantly in their susceptibility to V. anguillarum live cells. In contrast, the ECP from Vibrio strains of serotypes 01 and 02 exhibited similar lethal dose for turbot, salmon, and trout (ranging from 4.52 to 7.32 μg protein/g fish). Therefore, differences in susceptibility to vibriosis are not completely due to a differential sensitivity of fish to the extracellular products of Vibrio strains. The ECP from 7 of 10 V. anguillarum strains possessed vascular permeability factors, and all the extracts displayed proteolytic, hemolytic and cytotoxic activities. All the biological activities of ECP were lost after heat treatment at 80° C/10 min.