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UV‐irradiation of rainbow trout sperm as a practical method for induced gynogenesis
Author(s) -
Goryczko K.,
Dososz S.,
Mäkinen T.,
Tomasik L.
Publication year - 1991
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1991.tb00520.x
Subject(s) - rainbow trout , biology , sperm , serial dilution , insemination , andrology , irradiation , dilution , motility , artificial insemination , zoology , fish <actinopterygii> , fishery , genetics , pregnancy , alternative medicine , physics , pathology , nuclear physics , thermodynamics , medicine
Summary The goal of the experiment was to refine a simple and practical method for inducing gynogenesis in rainbow trout. To eliminate the male genome, UV‐irradiation was used in combination with seminal fluid dilution and continuous stirring. Sperm motility was controlled. A visible recessive marker (yellow colour) as well as the Hertwig Effect were applied to assess the outcome of induced gynogenesis. Sperm irradiation by UV (energy output 2075 μW/cm 2 ) for more than 5 minutes and 10 minutes in dilutions of seminal fluid of 1:40 and 1:20, respectively, resulted in yellow coloured feeding fry in the diploidized groups, whereas non‐viable larvae were produced in the haploid groups. The highest survival rate for gynogenomes (from insemination until first‐feeding fry) was 19 %.