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Comparison of the in vitro growth characteristics, viral polypeptides, and response to neutralizing monoclonal antibodies of nine isolates of infectious hematopoietic necrosis virus from Alaskan salmonids
Author(s) -
Eaton W. D.
Publication year - 1991
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1991.tb00516.x
Subject(s) - biology , virology , monoclonal antibody , in vitro , neutralizing antibody , virus , haematopoiesis , infectious hematopoietic necrosis virus , antibody , microbiology and biotechnology , necrosis , immunology , stem cell , fish <actinopterygii> , rainbow trout , fishery , genetics , biochemistry
Summary Nine isolates of infectious hematopoietic necrosis virus (IHNV) from three different species of Alaskan salmonids were examined for in vitro growth characteristics, the molecular weight of virion polypeptides, and serologic response to four different monoclonal antibodies developed against IHNV. The results of this study suggest that, although serologically similar, three antigenic variants of IHNV may be present in Alaska. All nine viruses were neutralized by the monoclonal antibody RB/B5. However, the viruses could be divided into three sub‐groups based on the ability or inability to react with the monoclonal antibodies 193–110/B4 and NR‐2. The growth characteristics of all nine viruses were similar enough to suggest there was no significant difference in phenotypes among these viruses. The molecular weights of the viral polypeptides were similar in that all had a glycoprotein (G) of 66 kd, a nucleocapsid (N) protein of 42 kd, ana a polymerase (L) of 180 kd molecular weight.