Premium
Rapid identification of Vibrio anguillarum by Colony hybridization
Author(s) -
Aoki Takashi,
Hirono Ikuo,
Castro Teresa,
Kitao Tadatoshi
Publication year - 1989
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1989.tb00475.x
Subject(s) - vibrio anguillarum , biology , plecoglossus altivelis , serotype , vibrio , microbiology and biotechnology , hybridization probe , dna , fish <actinopterygii> , genetics , bacteria , fishery
Summary A 562 base pair fragment of DNA from a serotype A strain of Vibrio anguillarum was cloned into pUC9 and used as a hybridization probe for the rapid identification of Vibrio anguillarum by colony hybridization. The probe was tested on nine different fish pathogens, 15 Vibrio isolates, 2 organisms closely related to Vibrio , and 9 serotypes of V. anguillarum. The probe hybridized only with the DNA of V. anguillarum serotypes A and H. The sequence of the 562 nucleotides have been determined. This probe allows rapid, reliable, and specific detection of V. anguillarum in freshwater ayu, Plecoglossus altivelis.