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Influence of sperm density and contact time on herring egg fertilization
Author(s) -
Rosenthal H.,
Klumpp D.,
Willführ J.
Publication year - 1988
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1988.tb00470.x
Subject(s) - human fertilization , biology , sperm , herring , clupea , fish <actinopterygii> , zoology , fishery , botany , anatomy
Summary In order to standardize fish egg incubation techniques for bioassay application, fertilization procedures need to be included into the protocols. Nothing is known about the necessary sperm concentrations required to achieve optimal fertilization rates. The tests described here for the herring Clupea harengus L. include trials with 10 different sperm densities and 4 contact times (15, 30, 60 and 120 seconds). The variability of fertilization rates in eggs from different females was also investigated. Fertilization success was mainly influenced by sperm density and less by the actual contact time between unfertilized eggs and sperm containingmedia. Dilution in sperm density to 9.6 × 10 6 cells ml ‐1 or less resulted in reduced fertilization success. There was considerable variability in fertilization rates between females.