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Monoclonal antibodies for a comparative serological study of strains of Vibrio anguillarum
Author(s) -
Goerlich R.
Publication year - 1987
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1987.tb00457.x
Subject(s) - biology , vibrio anguillarum , serotype , monoclonal antibody , serology , microbiology and biotechnology , vibrionaceae , vibrio , antibody , strain (injury) , hybridoma technology , antigen , virology , bacteria , immunology , genetics , anatomy
Summary Murine monoclonal antibodies against characteristic determinants of heat stable somatic antigens of typical serovar I (820/15/8 Kop) and serovar II (134/82/1 Kiel) of European Vibrio anguillarum strains were produced. Three stable hybridoma cell lines, called aVaI/1F4, aVaI/6F4 and aVaI/2H5, produced monoclonal antibodies each against a typical serovar I determinant of strain 820/15/8 Kop. One hybridoma cell line (aVaII/5A4) producing monoclonal antibodies against Vibrio anguillarum strain 134/82/1 Kiel (serovar. II) was established. Fourteen Vibrio strains and Aeromonas salmoniáda strains were serologically compared by Enzyme‐Linked Immunosorbent Assay (E lisa ).