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Detection of Renibacterium salmoninarum by the enzyme‐linked immunosorbent assay (ELISA)
Author(s) -
Dixon P. F.
Publication year - 1987
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1987.tb00456.x
Subject(s) - biology , microbiology and biotechnology , isolation (microbiology) , fish <actinopterygii> , bacteria , virology , fishery , genetics
Summary An enzyme‐linked immunosorbent assay (ELISA) was developed for the detection and identification of Renibacterium salmoninarum. The immune γ‐globulin used in the assay was absorbed with two species of cross‐reacting bacteria to make a specific test system. R. salmoninarum could be detected in clinically‐diseased fish within 30 minutes of preparing a kidney sample, and thus because of its ease of use, the ELISA could be employed as a rapid field test for bacterial kidney disease (BKD), although isolation of R. salmoninarum was more sensitive than the ELISA for detecting individual carrier fish.