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Detection of neutralizing antibody to Egtved virus in rainbow trout ( Salmo gairdneri ) by plaque neutralization test with complement addition
Author(s) -
Olesen N. J.,
Jørgensen P. E. Vestergård
Publication year - 1986
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1986.tb00427.x
Subject(s) - salmo , rainbow trout , biology , neutralization , trout , antibody , virology , neutralizing antibody , immune system , immunology , fish <actinopterygii> , fishery
Summary The humoral immune response of trout to Egtved virus (VHS) was examined by means of a 50 % plaque neutralization test with complement addition. The test, wich has been adapted for use in microtitre plates, is documented with regard to specificity and sensitivity. Eight weeks after VHS infection by cohabitation at 13 °C neutralizing antibody could be detected in 100% of individually tagged experimental trout. Maximal litres of 10240 were found six weeks after infection. In 36% of the trout antibody was still detectable one year after infection. Neutralizing antibody could be detected also in trout infected with VHS under trout farm conditions. The percentage of positive sera ranged from 4 to 56.