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Identification of two components of the female sex pheromone of the sugarcane‐borer Diatraea flavipennella (Lepidoptera: Crambidae)
Author(s) -
Kalinová B.,
Do Nascimento R. R.,
Hoskovec M.,
Mendonça A. L.,
Silva E. L.,
De Freitas M. R. T.,
CabralJr C. R.,
Silva C. E.,
Sant’Ana A. E. G.,
Svatoš A.
Publication year - 2012
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2011.01625.x
Subject(s) - crambidae , sex pheromone , pheromone , lepidoptera genitalia , biology , botany , stereochemistry , horticulture , chemistry
Analysis by gas chromatography with electroantennographic detection of extracts of pheromone glands derived from calling females of the sugarcane‐borer Diatraea flavipennella revealed two antennally active compounds. These components were identified as ( Z )‐9‐hexadecenal ( Z 9–16:Ald) and ( Z )‐11‐hexadecenal ( Z 11–16:Ald) by comparison of the retention times of the natural compounds and the synthetic compounds supported by two‐dimensional gas chromatography – time‐of‐flight mass spectrometric analysis and the positions of the double bounds in the chains were confirmed from the mass spectral fragmentation patterns of their dimethyldisulphide adducts. The analysis indicated that Z 9–16:Ald and Z 11–16:Ald were present in the sex pheromone in the proportions 25 : 75. Trace amounts of tetradecanal, hexadecanal, (Z) ‐7‐hexadecenal ( Z 7–16:Ald), (Z) ‐9‐hexadecen‐1‐ol and (Z) ‐11‐hexadecen‐1‐ol were also found in the extract, but of these only Z 9–16:Ald and Z11–16:Ald appeared to be antennally active. Behavioural bioassays demonstrated that a binary blend composed of Z 9–16:Ald and Z 11–16:Ald in the ratio of 25 : 75 induced a response in D. flavipennella virgin males similar to that elicited by live virgin females or by an hexane extract of the pheromone glands of calling females. Z 9–16:Ald and Z 11–16:Ald are, therefore, considered to be the major constituents of the female sex pheromone of D. flavipennella .