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The infectivity and host range of Orgyia anartoides nucleopolyhedrovirus
Author(s) -
Markwick N. P.,
Glare T. R.,
Hauxwell C.,
Li Z.,
Poulton J.,
Ward J. M.,
Young V. L.,
Ward V. K.
Publication year - 2010
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2009.01443.x
Subject(s) - biology , lepidoptera genitalia , tortricidae , noctuidae , plutellidae , larva , instar , infectivity , host (biology) , biological pest control , pest analysis , botany , veterinary medicine , zoology , ecology , virology , plutella , virus , diamondback moth , medicine
The painted apple moth (PAM), Teia anartoides (Walker) (Lepidoptera: Lymantriidae) made a recent incursion into New Zealand. A nucleopolyhedrovirus (NPV), Orgyia anartoides NPV ( Oran NPV), originally isolated from PAM in Australia, was tested for its pathogenicity to PAM and a range of non‐target insect species found in New Zealand, to evaluate its suitability as a microbial control for this insect invader. Dosage‐mortality tests showed that Oran NPV was highly pathogenic to PAM larvae; mean LT 50 values for third instars ranged from 17.9 to 8.1 days for doses from 10 2 to 10 5 polyhedral inclusion bodies/larva, respectively. The cause of death in infected insects was confirmed as Oran NPV. Molecular analysis established that Oran NPV can be identified by PCR and restriction digestion, and this process complemented microscopic examination of infected larvae. No lymantriid species occur in New Zealand; however, the virus had no significant effects on species from five other lepidopteran families (Noctuidae, Tortricidae, Geometridae, Nymphalidae and Plutellidae) or on adult honeybees. Thus, all indications from this initial investigation are that Oran NPV would be an important tool in the control of PAM in a future incursion of this species into New Zealand.