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Attraction of Mexican fruit flies (Diptera: Tephritidae) to bacteria: effects of culturing medium on odour volatiles
Author(s) -
Robacker D. C.,
Lauzon C. R.,
Patt J.,
Margara F.,
Sacchetti P.
Publication year - 2009
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2008.01364.x
Subject(s) - tephritidae , biology , petri dish , bacteria , food science , pantoea agglomerans , agar , odor , microbiology and biotechnology , uric acid , ammonia , chemically defined medium , inoculation , methyl eugenol , botany , biochemistry , horticulture , in vitro , genetics , neuroscience , pest analysis
Effects of culturing medium on production and emission of volatiles by Pantoea agglomerans (Beijerinck 1888) Gavini et al. 1989 preparations and on attractiveness of the preparations to the Mexican fruit fly, Anastrepha ludens Loew, were investigated. Bacterial cultures in each of four biochemically different types of liquid media emitted different volatiles. Cultures in a medium containing uric acid as its primary nitrogen source emitted more ammonia and 2‐nonanone than the other media. We postulate that the high production of ammonia was because of uricase activity by this uricase (+) strain. Regardless of media type, supernatants emitted more volatiles than preparations containing cells that had been removed from whole cultures and put into distilled water. Attractiveness varied little with biochemical make‐up of the culturing medium although the uric acid and carbohydrate preparations were as a group more attractive than preparations made from the other two media. Supernatants and whole cultures generally were more attractive than cell preparations and non‐inoculated media. Bacteria grown in aqueous uric acid‐based media emitted volatiles similar but not identical to those emitted by bacteria grown on gel (agar or solid) uric acid‐based media in Petri plates.