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Performance of marking techniques in the field and laboratory for Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae)
Author(s) -
Mattioli F.,
Cabrera Walsh G.
Publication year - 2008
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2007.01257.x
Subject(s) - biology , pigment , fluorescence , botany , toxicology , chemistry , organic chemistry , physics , quantum mechanics
A reliable marking technique was needed for a mark–release–recapture experiment with adults of Diabrotica speciosa (Germar). Four marking techniques, acrylic paint (spattered or brushed on the surface of the insect); and fluorescent pigments (dusted on surfaces or mixed with diet to produce an ingested marker), were tested. Fluorescent pigment durability for the dusting and ingested techniques was evaluated for laboratory conditions and under simulated field conditions. The impact of the techniques on beetle survival was also assessed. Both acrylic paint techniques caused mobility problems in the beetles, and neither technique lasted for more than 48 h. Both fluorescent pigment techniques were more reliable, but the dusting technique showed a significantly higher mortality than the control, and duration variations between laboratory and field conditions. Use of fluorescent pigments added to the diet was the most reliable technique. This technique allowed the manipulation of the marking period, and provided reliable timing of marker persistence in the field.