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Destruxin from Metarhizium anisopliae induces oxidative stress effecting larval mortality of the polyphagous pest Spodoptera litura
Author(s) -
Sree K. Sowjanya,
Padmaja V.
Publication year - 2008
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2007.01239.x
Subject(s) - metarhizium anisopliae , biology , spodoptera litura , superoxide dismutase , catalase , entomopathogenic fungus , oxidative stress , larva , pest analysis , spodoptera , microbiology and biotechnology , food science , antioxidant , lipid peroxidation , peroxidase , botany , toxicology , biological pest control , biochemistry , enzyme , beauveria bassiana , gene , recombinant dna
Nine‐day‐old Spodoptera litura (Fab.) larvae were treated with crude destruxin (dtx) extracted from a high‐virulent (M‐19) and a low‐virulent (M‐10) isolate of the entomopathogenic fungus, Metarhizium anisopliae (Metch.), at doses that caused 30%, 50% and 90% mortality in the treated groups after 48 h. Destruxins produced a dose‐dependent decrease in the body weight of the larvae after 1, 24 and 48 h of treatment. There was a considerable hike in the activity of lipoxygenase and lipid peroxidation levels in the treated larvae with increased time of exposure to mycotoxin. The activities of total superoxide dismutase, total catalase, total peroxidase and specific ascorbate peroxidase in the larval body also registered alterations in the dtx‐treated larvae, suggesting that exposure of larvae to crude dtx induces oxidative stress which is countered by the antioxidant enzymes to an extent governed by the concentration and time of treatment, beyond which the larvae succumb to the ecofriendly biotoxin.