Premium
Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real‐time PCR
Author(s) -
Feng Xian,
NaiZhong Chen,
Jun Ma,
Shuifang Zhu,
Xuenan Hu
Publication year - 2007
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2007.01216.x
Subject(s) - biology , agromyzidae , genbank , plant quarantine , polymerase chain reaction , quarantine , mitochondrial dna , botany , real time polymerase chain reaction , horticulture , pest analysis , genetics , gene , ecology
Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L . trifolii based on real‐time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L . trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L . trifolii ‐specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real‐time PCR assay system was 2 h, and it would save 3–7 h compared with conventional PCR.