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A simple method for the detection of imaginal diapause in beetles
Author(s) -
Doležal P.,
Sehnal F.
Publication year - 2007
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2006.01144.x
Subject(s) - leptinotarsa , biology , diapause , staining , insect , zoology , pest analysis , botany , larva , genetics
Investigations on Ips typographus and Leptinotarsa decemlineata revealed that the activity of mitochondrial enzymes in the flight muscles provides a reliable criterion for the discrimination of diapausing and non‐diapausing adults. Enzyme activity is conveniently detected in situ with commercially available tetrazolium salts. Staining intensity is correlated with the flight ability and often also with reproduction.