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Insecticidal activities of secondary metabolites of endophytic Pencillium sp. in Derris elliptica Benth
Author(s) -
Hu M. Y.,
Zhong G. H.,
Sun Zh. T.,
Sh G,
Liu H. M.,
Liu X. Q.
Publication year - 2005
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2005.00988.x
Subject(s) - rotenone , biology , bioassay , plutella , instar , high performance liquid chromatography , plant use of endophytic fungi in defense , chromatography , botany , column chromatography , toxicology , larva , chemistry , biochemistry , genetics , mitochondrion
A strain of endophytic Pencillium sp., which might produce rotenone or its analogues and showed bioactivity against aphids, was isolated from the fresh roots of Derris elliptica Benth. A total of 12 fractions, isolated from the chloroform extract of endophytic Pencillium sp. mycelia by silica gel column, were tested by bioassay and high‐performance liquid chromatography (HPLC), and the more bioactive fractions were found to be D, E and J. Against the adult turnip aphid, Lipaphis erysimi , by dipping at a concentration of 1 mg/ml, the corrected mortalities of fraction D, E and J were 57.68, 63.28 and 69.74% after 48 h of treatment respectively. The three fractions also showed strong antifeeding activity against third instar larvae of Plutella xylostella in a laboratory bioassay. One absorption peak was detected in the HPLC picture of fraction D, it had a similar retention time as that of rotenone, and the chemical constituent, related to the absoption peak, had the same ultraviolet absorption picture as that of rotenone. Then it could be further concluded that the bioactive compounds in the fraction D could be rotenone or its analogous compounds.