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Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences
Author(s) -
Raboudi F.,
Mezghani M.,
Makni H.,
Marrakchi M.,
Rouault J. D.,
Makni M.
Publication year - 2005
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2005.00934.x
Subject(s) - biology , aphis craccivora , myzus persicae , aphid , mitochondrial dna , cytochrome b , aphis gossypii , macrosiphum euphorbiae , zoology , aphididae , genetics , specific identification , botany , restriction fragment length polymorphism , gene , polymerase chain reaction , homoptera , pest analysis
In Tunisia, four major aphid species have been identified based on adult female's morphological characters: Aphis gossypii Glover, Aphis craccivora Koch, Myzus persicae Sluzer and Macrosiphum euphorbiae Thomas. Species identification at individual collection sites is often difficult because adults are much fewer in number than larvae which are not so easy to distinguish morphologically. We therefore set up an experiment to determine if cuticular hydrocarbon phenotypes and mitochondrial DNA haplotypes could be used to distinguish such sympatric species. Results showed that each species had an unique cuticular hydrocarbon phenotype and mitochondrial cytochrome b sequence. Cytochrome b restriction fragment‐length polymorphism markers, especially Dde I, identified in this sudy constitute a relatively simple and useful approach to distinguish the four species even at the nymphal stage.