Premium
Inhibition kinetics on carboxylesterase and acetylcholinesterase of Liposcelis bostrychophila and Liposcelis entomophila (Psocop., Liposcelididae) of two insecticides
Author(s) -
Cheng W.X.,
Wang J.J.,
Ding W.,
Zhao Z.M.
Publication year - 2004
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.2004.00846.x
Subject(s) - carbosulfan , acetylcholinesterase , paraoxon , biology , toxicology , biochemistry , enzyme , pesticide , agronomy
Abstract: The inhibition kinetics on carboxylesterase (CarE) and acetylcholinesterase (AChE) of Liposcelis bostrychophila and Liposcelis entomophila of paraoxon and carbosulfan were compared. The results showed that L. entomophila exhibited significantly greater specific activity of CarE than L. bostrychophila [0.045 vs. 0.012 μ mol of α ‐naphthyl acetate ( α ‐NA) hydrolysed/mg protein/min]. Moreover, L. entomophila showed higher affinity (i.e. lower K m value) to the substrate α ‐NA than L. bostrychophila (0.29 vs. 0.67 m m ). For AChE‐specific activity and affinity, no significant differences between the two species were observed. Based on the I 50 values, AChE of L. bostrychophila was more sensitive to paraoxon and carbosulfan than that of L. entomophila . According to inhibition kinetics, the results revealed that AChE of L. bostrychophila was 3.8‐fold more sensitive to inhibition by paraoxon than that of L. entomophila , but L. entomophila was 1.5‐fold more susceptible to carbosulfan.