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Production of Agrotis segetum granulosis virus in different larval instars of A. segetum (Lep., Noctuidae)
Author(s) -
VargasOsuna E.,
Carranza P.,
Aldebis H. K.,
SantiagoAlvarez C.
Publication year - 1995
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.1995.tb01360.x
Subject(s) - biology , instar , larva , noctuidae , virus , inoculation , moulting , veterinary medicine , botany , virology , horticulture , medicine
Virus yields from Agrotis segetum larvae infected in the third, fourth and fifth instar with an A. segetum granulosis virus (AsGV) were determined in terms of numbers of inclusion bodies. The number of inclusion bodies produced per larva was found to be inversely related to the instar of inoculated larvae. A greater yield of virus was obtained from larvae that had moulted after ingestion of virus inoculum than from those which died without moulting. Female larvae produced more virus than male larvae. The viruses obtained from third and fourth instar larvae had the highest regression line slopes with significant differences with respect to original inoculum. In general, the viral products were more virulent than the original inoculum but the differences were significant for viruses produced in larvae that died in the inoculation instar. The virus obtained from fifth instar larvae showed a significantly higher virulence when it was produced in male rather than female larvae.