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Effect of temperature on the development of baculoviruses
Author(s) -
Ribeiro H. C. T.,
Pavan O. H. O.
Publication year - 1994
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.1994.tb00806.x
Subject(s) - diatraea saccharalis , biology , trichoplusia , nuclear polyhedrosis virus , instar , larva , baculoviridae , zoology , inoculation , veterinary medicine , botany , horticulture , noctuidae , medicine , spodoptera , gene , recombinant dna , biochemistry
Development of infection of 4 strains of nuclear polyhedrosis virus (NPV) in third instar Diatraea saccharalis larvae at 10 different temperatures was compared. Two wild strains, the Anticarsia gemmatalis MNPV ( Ag MNPV) and the Trichoplusia ni MNPV ( Tn MNPV), and two selected strains, Ag MNPV‐D10 and Tn MNPV‐D11 were inoculated in 11‐day‐old D. saccharalis larvae and kept in temperatures of 17, 22, 24, 26, 28, 30, 32, 35, 37 and 39°C. The results showed that larval mortality increased with temperature, and the increase of temperature caused also the reduction of lethal time 50% (LT 50 ) values. The maximal polyhedral production was obtained at 28°C for the 4 viral strains and a drastic reduction in the number of polyhedra was observed even for a difference of 2°C above or below this ideal temperature. The selected strains showed a 10‐fold increase in polyhedra yield when compared to the wild type strains.