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Quick detection of resistant phenotypes with high esterase activity in two species of aphids (Homoptera, Aphididae)
Author(s) -
Marullo Rita,
Lövei G. L.,
Tallarico A.,
Tremblay E.
Publication year - 1988
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/j.1439-0418.1988.tb00585.x
Subject(s) - aphididae , biology , aphid , homoptera , esterase , botany , horticulture , pest analysis , biochemistry , enzyme
A qualitative filter paper test to determine total esterase activity in individual aphids was applied to sensitive and resistant strains of two aphid species selected in the laboratory and in the field. Increased esterase activity was found in two aphid species subjected to selection by two different types of insecticides, an organophosphorous compound and a carbamate. Sensitive aphids of boths species always gave faint spots while those of resistant ones were much darker. The test is simple and quick; it requires minimum equipment and can be performed under field conditions, too. Zusammenfassung Eine Methode zur raschen Unterscheidung zwischen insektizid‐sensitiven und ‐resistenten Individuen von zwei Blattlaus‐Arten (Hom., Aphididae) Es wurde ein qualitativer Test zur Bestimmung der Gesamt‐Esteraseaktivität bei insektizid‐ sensitiven und ‐resistenten Blattlausindividuen von Aphis pisum Harr. und Breviocoryne brassicae (L.) durchgeführt. Bei sensitiven Individuen beider Arten wies das Testpapier schwache, bei den resistenten Individuen dagegen starke Flecken auf. Der Test ist einfach und rasch, auch im Freiland, durchführbar.