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Zur Nahrungsqualität von Fichtennadeln für forstliche Schadinsekten
Author(s) -
Dinish K. N.
Publication year - 1974
Publication title -
zeitschrift für angewandte entomologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0044-2240
DOI - 10.1111/j.1439-0418.1974.tb03237.x
Subject(s) - chemistry , starch , amylase , enzyme , maltase , enzyme assay , substrate (aquarium) , microbiology and biotechnology , biochemistry , chromatography , food science , biology , ecology
As to food quality of spruce needles for forest damaging insects. 5. Studies on the starch splitting enzyme (Amylase) in larvae of Gilpinia hercyniae Htg . The starch splitting enzyme (Amylase) was demonstrated in larvae of Gilpinia hercyniae (Fig. 1). The biochemical properties such as substrate concentration, enzyme concentration, activation by Ca ++ ions, inhibition by EDTA, pH optimum and sensitivity to temperature (Fig. 2, Tab. 2) were studied. The reaction products due to enzyme action were separated by paper chromatography and compared with standards (Fig. 3). The variation of enzyme activity in 6, 12 and 18 days old larvae was studied (Fig. 4). The results with reference to the various conditions which influence the activity and its variation due to the age of larvae were discussed with respect to other carbohydrate metabolising enzymes such as maltase, gluco‐ and fructohexokinase. Zusammenfassung Das stärkespaltende Enzym (Amylase) wurde in Larven von Gilpinia hercyniae nachgewiesen und seine biochemischen Eigenschaften untersucht. Die Reaktionsprodukte nach Enzymeinwirkung wurden chromatographisch analysiert, die Abhängigkeit der Enzymaktivität vom Larvenalter nachgewiesen. Die Stellung des Enzyms im abbauenden Kohlenhydratstoffwechsel wird diskutiert.